35 μl of formamide-based hybridization buffer Search Results


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Summary of microarray and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analyses of <t> microarray-identified </t> transcripts
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Summary of microarray and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analyses of <t> microarray-identified </t> transcripts
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Summary of microarray and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analyses of <t> microarray-identified </t> transcripts
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Summary of microarray and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analyses of <t> microarray-identified </t> transcripts
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Summary of microarray and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analyses of <t> microarray-identified </t> transcripts
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Summary of microarray and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analyses of <t> microarray-identified </t> transcripts
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Summary of microarray and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analyses of <t> microarray-identified </t> transcripts
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Summary of microarray and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analyses of <t> microarray-identified </t> transcripts
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Summary of microarray and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analyses of <t> microarray-identified </t> transcripts
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Image Search Results


Summary of microarray and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analyses of  microarray-identified  transcripts

Journal: Journal of Fish Biology

Article Title: Identification of a gene set to evaluate the potential effects of loud sounds from seismic surveys on the ears of fishes: a study with Salmo salar

doi: 10.1111/jfb.12398

Figure Lengend Snippet: Summary of microarray and quantitative reverse transcription–polymerase chain reaction (qRT-PCR) analyses of microarray-identified transcripts

Article Snippet: The cDNA targets were mixed with 2 μl of LNA dT blocker, 3 μl of GFP spike and 35 μl of 2× formamide-based hybridization buffer (25% formamide, 4× SSC, 0·5% SDS, 2× Denhardt's solution) (Genisphere) and hybridized to the pre-warmed arrays by application of 22 mm × 60 mm HybriSlip coverslips (Grace Biolabs; www.gracebio.com ), in microarray hybridization chambers (Corning; www.corning.com ) in a 50° C water bath for 16 h in the dark.

Techniques: Microarray, Reverse Transcription Polymerase Chain Reaction, Sequencing, Quantitative RT-PCR